Saturated hydrogen alleviates CCl 4 -induced acute kidney injury via JAK2/STAT3/p65 signaling

Shujun Zhou, Song Wu, Zheng Fang

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DOI: 10.1177/0300060519895353 DOI is the universal ID for this study.

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Abstract:

Objectives: This study assessed the protective effects of saturated hydrogen against CCl4-induced acute kidney injury (AKI) in mice, and investigated signaling pathways activated by exposure to saturated hydrogen.

Methods: A mouse model of CCl4-induced AKI was established; some mice were treated with saturated hydrogen. Levels of cystatin C and kidney injury molecule 1 were determined using enzyme-linked immunosorbent assays. Blood urea nitrogen and serum creatinine were measured on a fully automated biochemical analyzer. Interleukin-8, tumor necrosis factor-α, and interferon-γ in serum and kidney tissues were measured using enzyme-linked immunosorbent assays. Malondialdehyde, glutathione peroxidase, and superoxide dismutase in kidney tissues were measured using biochemical kits. Oxidative stress in kidney tissues was analyzed using nitrotyrosine staining. Expression levels of p-JAK2, p-STAT3, and p-p65 signal protein were assayed by immunohistochemistry and western blotting.

Results: Compared with untreated mice with CCl4-induced AKI, mice that were treated with saturated hydrogen exhibited improved renal function and reduced oxidative stress. Moreover, expression levels of p-JAK2, p-STAT3, and p-p65 were significantly reduced in mice treated with saturated hydrogen, compared with expression levels in untreated mice. Conclusions: Treatment with saturated hydrogen can reduce oxidative stress and inflammatory cytokine activation, potentially through inhibition of JAK2/STAT3/p65 signaling, thereby protecting against AKI.


Publish Year 2020
Country China
Rank Positive
Journal Journal of International Medical Research
Primary Topic Kidney
Secondary TopicAcute Kidney Injury
Model Mouse
Tertiary TopicAcute Tubuluar Necrosis
Vehicle Saline (Dissolved)
pH Neutral
Application Injection
Comparison
Complement