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DOI:
10.1016/j.lfs.2021.119248
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Materials and methods: The rat model of myocardial ischemia-reperfusion was well established. Myocardial infarct size was evaluated by TTC & Evans blue staining. The no-reflow area and the cardiac function were assessed by thioflavin-S staining and echocardiography respectively. Microstructure and mitochondria of myocardial tissue were assessed by transmission electron microscope. Western blot and immunohistochemistry were used to evaluate the expression of NLRP3 mediated pyroptosis related proteins. The 8-OHdG, MDA and serum total ROS were used to evaluate the degree of oxidative stress. Key findings: The myocardial infarct size, no-reflow area, cardiac function, microstructure and mitochondrial morphology of I/R model rats were significantly improved after hydrogen inhalation. In addition, the expression of 8-OHdG, MDA, ROS and NLRP3 mediated pyroptosis related proteins were significantly decreased. Significance: We found that oxidative stress and NLRP3 mediated pyroptosis are the important mechanisms for hydrogen to alleviate myocardial I/R injury, and we also confirmed that hydrogen can significantly improve no reflow phenomenon caused by ischemia-reperfusion.
| Publish Year | 2021 |
|---|---|
| Country | China |
| Rank | Positive |
| Journal | Life Sciences |
| Primary Topic | Heart |
| Secondary Topic | Surgery/Transplantation |
| Model | Rat |
| Tertiary Topic | Ischemia-Reperfusion (I/R) Injury |
| Vehicle | Gas |
| pH | N/A |
| Application | Inhalation |
| Comparison | |
| Complement |