At a number of points in the current procedures of islet isolation and islet culture after the harvesting of donor pancreata, microorganisms could potentially infect the islet preparation. Furthermore, the use of islets from multiple donors can compound the risks of contamination of individual recipients. Acidic oxidative potential water (also termed electrolyzed strong acid solution, function ...
In the type 2 diabetes, it has become clear that reactive oxygen species (ROS) cause reduction of glucose uptake by inhibiting the insulin-signaling pathway in muscle cells and adipocytes. We demonstrated that electrolyzed-reduced water (ERW) scavenges ROS and protects DNA from oxidative damage1). Here we found that ERW scavenges ROS in insulin-responsive L6 myotubes and mouse3T3/L1 adipocytes. ...
The purposes of this study were to examine the time-related changes in pH, oxidation-reduction potential, and concentration of chlorine of electrolyzed neutral water and to evaluate the bactericidal effect of electrolyzed neutral water against bacteria from infected root canals. Various properties of electrolyzed neutral water--pH value, oxidation-reduction potential, and concentration of ...
We report on successful surgical treatment of consecutive three patients with infection of an abdominal aortic graft, Therapy involved a combination of removal of the infected graft, elaborate debridement, irrigation with electrolyzed strongly acidic aqueous solution (ESAAS), in-situ regrafting, and omentoplasty. The use of ESAAS is discussed.
Electrolyzed water accelerated the healing of full-thickness cutaneous wounds in rats, but only anode chamber water (acid pH or neutralized) was effective. Hypochlorous acid (HOCl), also produced by electrolysis, was ineffective, suggesting that these types of electrolyzed water enhance wound healing by a mechanism unrelated to the well-known antibacterial action of HOCl. One possibility is that ...
The purpose of the study was to determine whether electrolyzed oxidized water (EOW) functions as a bactericide in burn injury with Pseudomonas aeruginosa infection in a rat burn-wound model. Anesthetized Sprague-Dawley rats (n = 31) were subjected to third-degree burns to 30% of total body surface area. Two days after injury, all rats were infected with P. aeruginosa using 1 mL of a suspension ...
The purpose of the study was to understand the effectiveness of electrolyzed strong acid aqueous solution (ESAAS) for acceleration of epithelialization in a rat burn wound model. Eighteen anesthetized Sprague-Dawley rats received 30% total body surface area third-degree burns, and 2 days after injury, the wound eschars were removed. Rats were divided into 3 groups: Group 1, no irrigation; Group 2, ...
Biochemical decompression, a novel approach for decreasing decompression sickness (DCS) risk by increasing the tissue washout rate of the inert gas, was tested in pigs during simulated H2 dives. Since there is only limited physiological data on the use of H2 as a diving gas, direct calorimetry and respirometry were used to determine whether physiological responses to hyperbaric H2 and He are ...
During the 7.1-MPa hydrogen-helium-oxygen record human dive, we tested the hypothesis that the increased ambient pressure would alter the maximal muscle performance, specifically that breathing dense gas would lead to fatigue of the respiratory muscle. A group of hand muscles (adductor pollicis, AP) and the inspiratory muscles (IM) were studied in three professional divers. Maximal voluntary ...
Electrolyzed reduced water and natural waters such as Hita Tenryosui water in Japan, Nordenau water in Germany and Tracote water in Mexico, which are known to improve various diseases, were all and- oxidative waters which could scavenge intracellular reactive oxygen species. The and-oxidative waters stimulated not only the glucose uptake of rat myotube L6 cells, but also the secretion of insulin ...
We previously reported that body weight on day 14 after birth in male offspring of rats given alkaline ionized water (AKW) was significantly heavier than that in offspring of rats given tap water (TPW), but no significant difference was noted in milk yield and in suckled milk volume between the two groups. Additionally, the offspring in the AKW group and TPW group were given AKW and TPW, ...
We investigated the suppressive effect of electrolyzed reduced water (ERW) on the growth of not only various human cancer cells but also microorganisms such as gram-negative Escherichia coli. ERW suppressed the growth of cancer cells, especially in soft-agar culture. The suppressive effect of ERW on the growth of cancer cells depended upon cell types and malignancy of cancer cells and the ...
Antioxidant vitamins and enzymes such as superoxide dismutase, catalase and glutathione peroxidase are considered to function as scavengers against reactive oxygen species and to provide protection against reactive oxygen species, including free radicals. Although antioxidants such as L-ascorbic acid, d-catechin and quercetin dehydrate show superoxide dismutation activity, using reduced water ...
Electroly-reduced water (ERW) which is produced near cathode during electrolysis of water scavenges reactive oxygen species and protects DNA from oxidative damage (Shirahata et al., 1997). Most of cancer cells exhibit high telomerase activity to elongate telomere length, insuring their immortality. Here we found that cultivation of human lung adenocarciomaA549, human uterine cancer HeLaand human ...
Molecular hydrogen reacts with the hydroxyl radical, a highly cytotoxic species produced in inflamed tissues. It has been suggested therefore to use gaseous hydrogen in a new anti-inflammatory strategy. We tested this idea, with the aid of the equipment and skills of COMEX SA in Marseille, a group who experiments with oxygen-hydrogen breathing mixtures for professional deep-sea diving. The model ...
Neutrophils activation and tumor necrosis factor-α (TNF-α) induction play critical roles in aspirin-induced gastric mucosal injury. The aim of the present study was to determine whether electrolyzed alkaline water (EAW) can ameliorate aspirin-induced gastric mucosal injury in rats, and whether EAW can inhibit the increased gastric TNF-α expression associated with neutrophil accumulation and ...
The study examined changes in the test parameters of 101 diabetes patients (56 female, 45 male) under the influence of what is known as the “Nordenau Phenomenon”, which attributes a healing effect to an old slate tunnel with an underground spring. The average age of the test persons was 67,9 years; the average duration of their stay in Nordenau was 3 days for group 1 (61 patients), ...
Reactive oxygen species (ROS) cause irreversible damage to biological macromolecules, resulting in many diseases. Reduced water (RW) such as hydrogen-rich electrolyzed reduced water and natural reduced waters like Hita Tenryosui water in Japan and Nordenau water in Germany that are known to improve various diseases, could protect a hamster pancreatic beta cell line, HIT-T15 from alloxan-induced ...
The study is designed to investigate bactericidal actions of electrolyzed oxidizing water on hospital infections. Ten of the most common opportunistic pathogens are used for this study. Cultures are inoculated in 4.5 mL of electrolyzed oxidizing (EO) water or 4.5 mL of sterile deionized water (control), and incubated for 0, 0.5, and 5 min at room temperature. At the exposure time of 30 s the EO ...
Objective: To study the efficacy of electrolyzed oxidizing water (EOW) and hydrocolloid occlusive dressings in the acceleration of epithelialization in excised burn-wounds in rats. Methods: Each of the anesthetized Sprague-Dawley rats (n=28) was subjected to a third-degree burn that covered approximately 10% of the total body surface area. Rats were assigned into four groups: Group I (no ...
Electrolyzed reduced water (ERW) is known to scavenge reactive oxygen species (ROS) which is related to malignant tumor phenotypes. We attempted to clarify the effect of ERW on tumor phenotypes of K562 human leukemia cells. Treatment of K562 cells with ERW resulted in growth arrest, morphological changes, and expression of CD41, a cell marker of megakaryocyte differentiation. However, the ...
The aim of this study was to assess the adequacy of our treatment strategy for patients with post-sternotomy mediastinitis. Between May 1997 and December 2000, 1,045 consecutive adult cardiac operations were performed at our center. Mediastinitis occurred in 8 patients (0.77%) and as treatment, they underwent (1) aggressive debridement, (2) closed irrigation and drainage, and (3) transvenous ...
Water-electrolysis with ion-exchange membranes followed by passing through ac- tivated carbon filter conducted to produce electrolyzed-reduced water that has neutral and reductive characteristics. In addition, the electrolyzed-reduced water was also confirmed characteristic of antioxidative activity. Bathing in the electrolyzed-reduced water lowered the ORP (oxidation-reduction potential) of human ...
Background: Increased oxidative stress in end-stage renal disease (ESRD) patients may oxidize macromolecules and consequently lead to cardiovascular events during chronic hemodialysis. Electrolyzed reduced water (ERW) with reactive oxygen species (ROS) scavenging ability may have a potential effect on reduction of hemodialysis-induced oxidative stress in ESRD patients. Methods: We developed a ...