Oxidative stress is produced under diabetic conditions and is likely involved in progression of pancreatic beta-cell dysfunction found in diabetes. Both an increase in reactive oxygen free radical species (ROS) and a decrease in the antioxidant defense mechanism lead to the increase in oxidative stress in diabetes. Electrolyzed reduced water (ERW) with ROS scavenging ability may have a potential ...
The generation of reactive oxygen species is thought to cause extensive oxidative damage to various biomolecules such as DNA, RNA, and protein. In this study, the preventive, suppressive, and protective effects of in vitro supplementation with electrolyzed-reduced water on H2O2-induced DNA damage in human lymphocytes were examined using a comet assay. Pretreatment, cotreatment, and posttreatment ...
This study investigated the antibacterial effect of electrolyzed water on oral bacteria both in vitro and in vivo. Tap water was electrolyzed in a water vessel using platinum cell technology. The electrolyzed tap water (called Puri-water) was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. In addition, Puri-water was used as a ...
According to the two-stage cell transformation theory, cancer cells first receive initiation, which is mainly caused by DNA damage and then promotion, which enhance transformation. Since murine Balb/c 3T3 cells lose contact inhibition by cell transformation, the cells have been widely used for transformation experiments. Electrolyzed reduced water (ERW) is a health beneficial alkaline drinking ...
The purpose of this study was to evaluate the cleaning effect of root canal walls using strong acid electrolytic water (SAEW) as a root canal irrigant, and to investigate the influence of SAEW on the root canal dentin by micro-hardness test. Forty-three single-rooted, single-canaled teeth were instrumented using standard step-back technique with K-files. Irrigation was performed using distilled ...
We have proposed an active hydrogen reduced water theory that active hydrogen produced by electrolysis of water is stabilized in the form of hydrogenated metal nanocolloids in electrolyzed reduced water (ERW) and scavenges intracellular reactive oxygen species (ROS). Because various brain diseases are caused by oxygen stress, we examined the effect of ERW on oxidative stress-induced apoptois of ...
Recent studies have demonstrated that reactive oxygen species9ROS9and the resulting oxidative stress play an important role in apoptosis. Apoptosis is implicated in pathophysiology of diabetes mellitus. Antioxidants can block or delay apoptosis. We have demonstrated that reduced water (RW) such as hydrogen-rich electrolysed-reduced water (ERW) and natural reduced waters (NRW) like Hita Tenryosui ...
In the two-stage cell transformation theory, cancer cells first receive initiation, which is mainly caused by DNA damage, and then promotion, which enhances transformation. Murine Balb/c 3T3 cells are widely used for transformation experiments because they lose contact inhibition ability when transformed. Electrolyzed reduced water (ERW), which is produced near a cathode during electrolysis of ...
A new type of electrolyzed hydrogen-saturated (EHS) water was produced using a water-electrolyzing device equipped with a special cation exchanger. Use of the EHS water for drinking in a feeding test with rats elicited an antioxidative effect. After intraperitoneal injection of 2,2-azobis-amidinopropane dihydrochloride, urinary secretion of 8-hydroxydeoxyguanosine and hepatic formation of ...
The purpose of this study was to determine the efficacy and the tolerability of the Charme™ system, a hand-held device that electrolyzes a water-based sodium chloride solution and delivers electrolyzed oxidized water (EOW) to the skin, in the treatment of mild to moderate acne. A total of 31 patients with mild to moderate acne vulgaris were enrolled in this 8-week, open-label, split-face pilot ...
We reported that reduced water produced by electrolysis enhanced the antioxidant effects of proton donors such as ascorbic acid (AsA) in a previous paper. We also demonstrated that reduced water produced by electrolysis of 2 mM NaCl solutions did not show antioxidant effects by itself. We reasoned that the enhancement of antioxidant effects may be due to the increase of the ionic product of water ...
We evaluated the properties and real existence of an electrolyzed-reduced water, which we prepared, and three commercially purchased water goods, that are advertised to have antioxidant activities by the action of 'active hydrogen,' on the basis of the results of examinations for inhibitory effects on the oxidative reactions of biomolecules, quantitative analyses of the minerals, and the ESR ...
We examined the suppressive effect on lipid peroxidation of electrolyzed reduced water (ERW), which exhibited reactive oxygen species (ROS)-scavenging activity in vitro and in cultured cells. The oxidation of linoleic acid by 2, 2’-azobis (2-amidinopropane) hydrochloride (AAPH) was suppressed by ERW in vitro. ERW also suppressed the lipid peroxidation in cell membrane of mouse lymphoblast YAC-1 ...
Background: Increased oxidative stress in end-stage renal disease (ESRD) patients may oxidize macromolecules and consequently lead to cardiovascular events during chronic hemodialysis. Electrolyzed reduced water (ERW) with reactive oxygen species (ROS) scavenging ability may have a potential effect on reduction of hemodialysis-induced oxidative stress in ESRD patients. Methods: We developed a ...
Electrolyzed-reduced water (ERW) produced by electrolyzing water in cathode side has the ability to scavenge reactive oxygen species (ROS). Here, we investigated the effect of ERW on oxidative stress-induced neural cell death by glutamate. When cell viability assay was performed using primary rat cerebral cortical culture as neural model, ERW suppressed neural cell death by glutamate. Furthermore, ...
Electrolyzed reduced water (ERW) is known to scavenge reactive oxygen species (ROS) which is related to malignant tumor phenotypes. We attempted to clarify the effect of ERW on tumor phenotypes of K562 human leukemia cells. Treatment of K562 cells with ERW resulted in growth arrest, morphological changes, and expression of CD41, a cell marker of megakaryocyte differentiation. However, the ...
The aim of this study was to assess the adequacy of our treatment strategy for patients with post-sternotomy mediastinitis. Between May 1997 and December 2000, 1,045 consecutive adult cardiac operations were performed at our center. Mediastinitis occurred in 8 patients (0.77%) and as treatment, they underwent (1) aggressive debridement, (2) closed irrigation and drainage, and (3) transvenous ...
Water-electrolysis with ion-exchange membranes followed by passing through ac- tivated carbon filter conducted to produce electrolyzed-reduced water that has neutral and reductive characteristics. In addition, the electrolyzed-reduced water was also confirmed characteristic of antioxidative activity. Bathing in the electrolyzed-reduced water lowered the ORP (oxidation-reduction potential) of human ...
The study is designed to investigate bactericidal actions of electrolyzed oxidizing water on hospital infections. Ten of the most common opportunistic pathogens are used for this study. Cultures are inoculated in 4.5 mL of electrolyzed oxidizing (EO) water or 4.5 mL of sterile deionized water (control), and incubated for 0, 0.5, and 5 min at room temperature. At the exposure time of 30 s the EO ...
Objective: To study the efficacy of electrolyzed oxidizing water (EOW) and hydrocolloid occlusive dressings in the acceleration of epithelialization in excised burn-wounds in rats. Methods: Each of the anesthetized Sprague-Dawley rats (n=28) was subjected to a third-degree burn that covered approximately 10% of the total body surface area. Rats were assigned into four groups: Group I (no ...
The study examined changes in the test parameters of 101 diabetes patients (56 female, 45 male) under the influence of what is known as the “Nordenau Phenomenon”, which attributes a healing effect to an old slate tunnel with an underground spring. The average age of the test persons was 67,9 years; the average duration of their stay in Nordenau was 3 days for group 1 (61 patients), ...
Reactive oxygen species (ROS) cause irreversible damage to biological macromolecules, resulting in many diseases. Reduced water (RW) such as hydrogen-rich electrolyzed reduced water and natural reduced waters like Hita Tenryosui water in Japan and Nordenau water in Germany that are known to improve various diseases, could protect a hamster pancreatic beta cell line, HIT-T15 from alloxan-induced ...
Neutrophils activation and tumor necrosis factor-α (TNF-α) induction play critical roles in aspirin-induced gastric mucosal injury. The aim of the present study was to determine whether electrolyzed alkaline water (EAW) can ameliorate aspirin-induced gastric mucosal injury in rats, and whether EAW can inhibit the increased gastric TNF-α expression associated with neutrophil accumulation and ...
Electroly-reduced water (ERW) which is produced near cathode during electrolysis of water scavenges reactive oxygen species and protects DNA from oxidative damage (Shirahata et al., 1997). Most of cancer cells exhibit high telomerase activity to elongate telomere length, insuring their immortality. Here we found that cultivation of human lung adenocarciomaA549, human uterine cancer HeLaand human ...
Molecular hydrogen reacts with the hydroxyl radical, a highly cytotoxic species produced in inflamed tissues. It has been suggested therefore to use gaseous hydrogen in a new anti-inflammatory strategy. We tested this idea, with the aid of the equipment and skills of COMEX SA in Marseille, a group who experiments with oxygen-hydrogen breathing mixtures for professional deep-sea diving. The model ...