Bactericidal effect of electrolyzed neutral water on bacteria isolated from infected root canals

Naoki Horiba, Kouiti Hiratsuka, Takaya Onoe, Tsutomu Yoshida, Kazuyoshi Suzuki, Toru Matsumoto, Hiroshi Nakamura

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DOI: 10.1016/S1079-2104(99)70300-8 DOI is the universal ID for this study.

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The purposes of this study were to examine the time-related changes in pH, oxidation-reduction potential, and concentration of chlorine of electrolyzed neutral water and to evaluate the bactericidal effect of electrolyzed neutral water against bacteria from infected root canals. Various properties of electrolyzed neutral water--pH value, oxidation-reduction potential, and concentration of chlorine--were measured at different times after storage of the water in the open state, the closed state, or the closed-and-dark state. The bactericidal effect of the various electrolyzed neutral water samples was then tested against 17 strains of bacteria, including 15 strains isolated from infected canals, as well as against 1 strain of fungus. Each bacterial or fungal suspension was mixed with electrolyzed neutral water, and the 2 substances were reacted together for 1 minute. After incubation for 1 to 7 days, the bactericidal effect of the electrolyzed neutral water was determined. The pH value and oxidation-reduction potential of electrolyzed neutral water remained almost unchanged when the water was stored in a dark, closed container. However, the concentration of chlorine decreased from 18.4 ppm to 10.6 ppm. Electrolyzed neutral water showed a bactericidal or growth-inhibitory effect against the bacteria. The results indicate that electrolyzed neutral water maintains a constant pH and oxidation-reduction potential when kept in a closed container without light and that it exhibits a bacteriostatic/bactericidal action against isolates obtained from infected root canals.

Publish Year 1999
Country Japan
Rank Positive
Journal Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
Primary Topic Mouth
Secondary Topic Dentin Integrity
Model Cell Culture
Tertiary Topic Root Canal
Vehicle Water (Electrolysis)
pH Neutral
Application Culture Media