Translational Research of Peritoneal Dialysis Solution with Dissolved Molecular Hydrogen

Masaaki Nakayama, Kimio Watanabe, Yoshimitsu Hayashi, Hiroyuki Terawaki, Wan Zhu, Shigeru Kabayama, Sadayoshi Ito

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DOI: 10.1159/000485717 DOI is the universal ID for this study.

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Abstract:

Background: Improved biocompatibility of peritoneal dialysis solution (PDS) is crucial for peritoneal membrane preservation, thereby ensuring long-term peritoneal dialysis (PD) and preventing encapsulating peritoneal sclerosis. We previously reported the protective effect of molecular hydrogen (H2) on mesothelial cells from PDS in nonuremic rats. Summary: In the present study, we examined the effect of H2-containing PDS (commercially available neutral pH type) regarding the protection of peritoneal tissue in experimental chronic kidney disease rats. Furthermore, we conducted a 2-week clinical trial in which H2-containing PDS was used in place of standard PDS and its feasibility was examined. In the experimental study, test solutions were injected through the subcutaneous port into the abdomen for 3 weeks. Histological study revealed a significant increase in the number of mesothelial cells and a significant decrease in peritoneal thickness in the H2-PD group as compared to the control and PD groups. Also, results of immunostaining analysis revealed increased vimentin and apoptotic cells in the membrane of the PD group, indicating that H2 may play a role in ameliorating PDS-induced peritoneal injury and preserving peritoneal integrity. In the clinical trial with 6 prevalent PD patients, all subjects completed the study with no adverse effects. Moreover, there were substantial changes in surrogate markers, such as increased CA125 and mesothelin, in the effluent in selected cases, suggesting enhanced mesothelial regeneration by H2. Key Message: H2-enriched PDS is a candidate novel PDS with improved biocompatibility. Further, our results support the significance of H2-PD clinical trials in the future.

Publish Year 2018
Country Japan
Rank Positive
Journal Contributions to Nephrology
Primary Topic Peritonium
Secondary Topic Peritoneal Dialysis
Model Human
Tertiary Topic Biocompatibility
Vehicle Solution (Dissolved)
pH Alkaline
Application Injection
Comparison
Complement