Protective effects of hydrogen-rich saline on monocrotaline-induced pulmonary hypertension in a rat model

Yun Wang, Lei Jing, Xiao Zhao, Ji Han, Zuo Xia, Shu Qin, Ya Wu, Xue Sun

Read more:

DOI: 10.1186/1465-9921-12-26 DOI is the universal ID for this study.

This link will take you to the full study.

Abstract:

Hydrogen-rich saline has been reported to have antioxidant and anti-inflammatory effects and effectively protect against organ damage. Oxidative stress and inflammation contribute to the pathogenesis and/or development of pulmonary hypertension. In this study, we investigated the effects of hydrogen-rich saline on the prevention of pulmonary hypertension induced by monocrotaline in a rat model. In male Sprague-Dawley rats, pulmonary hypertension was induced by subcutaneous administration of monocrotaline at a concentration of 6 mg/100 g body weight. Hydrogen-rich saline (5 ml/kg) or saline was administered intraperitoneally once daily for 2 or 3 weeks. Severity of pulmonary hypertension was assessed by hemodynamic index and histologic analysis. Malondialdehyde and 8-hydroxy-desoxyguanosine level, and superoxide dismutase activity were measured in the lung tissue and serum. Levels of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-6) in serum were determined with enzyme-linked immunosorbent assay. Hydrogen-rich saline treatment improved hemodynamics and reversed right ventricular hypertrophy. It also decreased malondialdehyde and 8-hydroxy-desoxyguanosine levels, and increased superoxide dismutase activity in the lung tissue and serum, accompanied by a decrease in pro-inflammatory cytokines. These results suggest that hydrogen-rich saline ameliorates the progression of pulmonary hypertension induced by monocrotaline in rats, which may be associated with its antioxidant and anti-inflammatory effects.

Publish Year 2011
Country China
Rank Positive
Journal Respiratory Research
Primary Topic Heart
Secondary Topic Hypertension
Model Rat
Tertiary Topic Oxidative Stress
Vehicle Saline (Dissolved)
pH Neutral
Application Injection
Comparison
Complement