Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice
Read more:
DOI:
10.1016/j.bbrc.2008.08.020
DOI is the universal ID for this study.
This link will take you to the full study.
Abstract:
Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H(2)) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H(2), VC, or pure water (H(2)O), administered for 33 days. VC levels in H(2) and H(2)O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H(2) group subjected to ischemia-reperfusion than in the H(2)O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.| Publish Year | 2008 |
|---|---|
| Country | Japan |
| Rank | Positive |
| Journal | Biochemical and Biophysical Research Communications |
| Primary Topic | Brain |
| Secondary Topic | ROS-Scavenging |
| Model | Mouse |
| Tertiary Topic | Oxidative Stress |
| Vehicle | Water (Dissolved) |
| pH | Neutral |
| Application | Ingestion |
| Comparison | Vitamin C |
| Complement |