Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice

Yasunori Sato, Shizuo Kajiyama, Akiko Amano, Yoshitaka Kondo, Toru Sasaki, Setsuko Handa, Ryoya Takahashi, Michiaki Fukui, Goji Hasegawa, Naoto Nakamura, Hikohito Fujinawa, Toyotaka Mori, Mitsuhiro Ohta, Hiroshi Obayashi, Naoki Maruyama, Akihito Ishigami

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DOI: 10.1016/j.bbrc.2008.08.020 DOI is the universal ID for this study.

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Abstract:

Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H(2)) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H(2), VC, or pure water (H(2)O), administered for 33 days. VC levels in H(2) and H(2)O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H(2) group subjected to ischemia-reperfusion than in the H(2)O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.

Publish Year 2008
Country Japan
Rank Positive
Journal Biochemical and Biophysical Research Communications
Primary Topic Brain
Secondary Topic ROS-Scavenging
Model Mouse
Tertiary Topic Oxidative Stress
Vehicle Water (Dissolved)
pH Neutral
Application Ingestion
Comparison Vitamin C
Complement